Rutgers ORA

Office of Research and Economic Development (ORED)

Chromatography

Department : Environmental and Occupational Health Sciences Institute

IC systems differ from HPLC systems having pump heads coated with Teflon to prevent corrosion; they can pump very acid or very basic solutions.  These systems are used for speciation by separating in time each chemical form of an analyte and the ICPMS is used as the chromatographic detector.

Department : Environmental and Occupational Health Sciences Institute

The LC pump can handle the widest array of gradient conditions and mobile phase switching.  It has been dedicated to the analysis of pharmaceutical analytes requiring changes in both gradient and ionization polarity and is a more sensitive instrument than the LCQ.

Department : Rutgers Center for Lipid Research, in the New Jersey Institute for Food, Nutrition, and Health

The Nutritional Metabolomics Core Facility was established to provide researchers with data on the identities and amounts of small molecules in biological samples. 

The facility specializes in the analysis of lipids, including phospholipids, cholesterol and cholesteryl esters, acyl coAs, endocannabinoids, and mono-, di-, and triacylglycerols.  Often, data for multiple lipid classes can be obtained from a single sample injection.  We plan to increase our offerings to include other metabolite classes as we expand into a full-fledged metabolomics laboratory.

Department : New Jersey Institute for Food, Nutrition, and Health

The ABSciex 4000QTrap is a hybrid triple quadrupole-linear ion trap mass spectrometer with a variety of available scan modes.  ESI, APCI, and APPI ion sources are available.  We find that the ESI and APPI sources complement each other well; the APPI source better ionizes several lipid classes that are not ionized as well by ESI.  The system is interfaced with a Dionex UltiMate 3000 liquid chromatograph with ternary gradient capabilities, temperature-controlled autosampler, thermostatted column oven, and PDA detector.  

Department : Newark College of Arts and Sciences - Department of Chemistry

The Apex-ultra 70 hybrid fourier transform mass spectrometer (FTMS) - Bruker Daltonics - is equipped with a 7.0 T actively shielded, high-stability magnet, and an Apollo II Dual ESI/MALDI source to provides simultaneous operation in both modes. The ESI source has an ion funnel and a spray chamber with a grounded off-axis sprayer. The MALDI source has computer controlled X-Y stage for scout-385 MTP target plates. Additional instrument capabilities are HPLC and nanospray experiments.